Inventors: Ruan H, Zhou CW, Dilaremu, Xu J, Wang RZ, Lin JH, He GQ
Patent Title: Method for performing catalytic synthesis of maltose stearate by using yeast display lipase
Publication date: 2011
Publication number: CN102212593

Abstract: The invention discloses a method for performing catalytic synthesis of maltose stearate by using yeast display lipase. The method comprises the following steps: dissolving maltose and stearic acid in organic solvent, adding yeast display lipase, reacting at 50-60 DEG C for 10-14 hours, separating, and purifying to prepare maltose stearate. The preparation method of yeast display lipase comprises the following steps: delivering the linearized recombinant plasmid in Pichia pastoris GS115, inoculating the obtained transformants in BMMY culture medium, inducing and culturing for 72-144 hours, centrifuging to collect bacteria, washing the bacteria, and performing bioimprinting and freeze drying to prepare yeast display lipase. In the method, lipase is displayed outside cells and the enzyme preparation is utilized to perform the catalytic synthesis of maltose stearate, thus the conversion efficiency can be increased, the reaction time can be shortened and the production cost can be reduced.
URL: http://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20111012&DB=EPODOC&locale=en_EP&CC=CN&NR=102212593A&KC=A&ND=4