Inventors: Ruan H, Xu J, Dilireba, Zhou CW, Wang RZ, Lin JH, He GQ
Patent Title: Method for performing catalytic synthesis of vitamin A palmitate by using yeast display lipase
Publication date: 2011
Publication number: CN102212601

Abstract: The invention discloses a method for performing catalytic synthesis of vitamin A palmitate by using yeast display lipase. The method comprises the following steps: dissolving vitamin A acetate and palmitic acid in organic solvent, adding yeast display lipase, reacting at 50-60 DEG C for 10-14 hours under the anaerobic condition, separating, and purifying to prepare vitamin A palmitate. The preparation method of yeast display lipase comprises the following steps: delivering the linearized recombinant plasmid in Pichia pastoris GS115, inoculating the obtained transformants in BMMY culture medium, inducing and culturing for 72-144 hours, centrifuging to collect bacteria, washing the bacteria, and performing bioimprinting and freeze drying to prepare yeast display lipase. In the method, lipase is displayed outside cells and the enzyme preparation is utilized to perform the catalytic synthesis of vitamin A palmitate, thus the conversion efficiency can be increased, the reaction time can be shortened and the production cost can be reduced.
URL: http://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20111012&DB=EPODOC&locale=en_EP&CC=CN&NR=102212601A&KC=A&ND=4