Inventors: Lin HY
, Lee MH
, Chen YL
A fabrication method of molecularly imprinted polymeric thin film coated nanostructure biosensor for the examination of urinary biomarkers
Publication date: 2014
In this invention, ceramic nanostructures were selectively hydrothermally grown on acetate-seeded spots on a gold substrate. Urinary biomarkers were imprinted into polymers, which were coated onto ceramic nanostructures. The ceramic nanostructures not only increased the surface area of sensing for recognizing target molecules, but also constitute an optical or electrochemical sensing element, as the fluorescence decreases when targets bind to the imprinted polymeric film. In real urine analysis, the molecularly imprinted polymeric (MIP) films responded linearly to added urinary biomarker, even in the presence of possible interfering compounds. This demonstrates the feasibility of using these MIPs as part of a total urinalysis MIP system.
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