Inventors: Zhang YD, Ma KK, Xu JX, Yue XF, Zhang L, Li MF, Wang LH
Patent Title: Method for preparing mimic enzyme molecularly imprinted polymer microsphere for hydrolyzing organophosphorus
Publication date: 2014
Publication number: CN104140494

Abstract: The invention discloses a method for preparing a mimic enzyme molecularly imprinted polymer microsphere for hydrolyzing organophosphorus. A paraoxon transient state analogue (4-nitro benzyl) phosphoric acid diethyl ester serves as a template molecule, 1-vinyl imidazole and methacrylic acid are functional monomers, a molecular imprinting technology is used, a precipitation polymerization method is adopted, and therefore the mimic enzyme molecularly imprinted polymer microsphere for hydrolyzing the organophosphorus is prepared, wherein the mimic enzyme molecularly imprinted polymer microsphere for hydrolyzing the organophosphorus is good in dispersibility and uniform in particle size. The paraoxon hydrolytic enzyme activity of the prepared molecularly imprinted polymer microsphere and the paraoxon spontaneous hydrolyzing are compared, the hydrolyzing efficiency can be improved by 188 times to the maximum and is also improved by 2.3 times compared with the catalyzing efficiency of a non-imprinted polymer synthesized under the condition of not adding template molecules, a measured Km value and a measured Vmax value are 0.064 mmol/L min and 2.41 mmol/L respectively, and Kcat is 0.237/S. The prepared mimic enzyme molecularly imprinted polymer microsphere for hydrolyzing the organophosphorus can be used for efficiently hydrolyzing organophosphorus pesticide and can also be used for degrading and destroying organophosphorus nervous toxic agent chemical weapons.
URL: http://worldwide.espacenet.com/publicationDetails/biblio?DB=EPODOC&II=0&ND=3&adjacent=true&locale=en_EP&FT=D&date=20141112&CC=CN&NR=104140494A&KC=A