Inventors: Tai DF
, Lin CY
, Wu TZ
Coating of molecularly imprinted membranes on chips to recognize biomolecules
Publication date: 2004
Based on the direct formation of molecularly imprinted membrane on gold electrode, we have developed an immunosensor for the detection of biomolecules. A new cross-linking monomer, (N-Acr-L-Cys-NHBn)2 was employed to attach the surface of the chip and to copolymerize with other monomers. Using quartz crystal microbalance detection, peptides and virus proteins can be monitored by their interaction with plastic antibodies specific for the target peptides. The selectivity of molecularly imprinted polymer and the sensitivity of such artificial biosensors have collaborated to differentiate traces of peptides and proteins to the ng/ml scale. Molecularly imprinted membranes also demonstrate the high ability to reuse.
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