Inventors: Chen DL, Zhang Y, Liu LJ, He LR
Patent Title: Preparation method and application of hybrid peptide with high arginine content in cervical cancer treatment
Publication date: 2018
Publication number: CN107574209A

Abstract: The invention relates to a hybrid peptide with a high arginine content and an application thereof in cervical cancer treatment. The preparation method comprises the following steps: using an ultrahighpressure to preprocess walnut meal and egg-white protein suspension; using alkaline protease and papain to enzymatically hydrolyze the preprocessing product in steps under the assistance of ultrasonic waves and microwaves; filtering the enzymatic hydrolysate, subjecting obtained supernate to reversed phase high performance liquid chromatography so as to separate target hybrid peptides in the supernate; taking the hybrid peptides as the template molecule, taking acrylic acid and methacrylic acid as the functional monomers, taking triethylene glycol dimethacrylate as the crosslinking agent, taking isopropyl thioxanthone as the initiator, and carrying out polymerization triggered by UV rays to form a surface molecularly imprinted membrane, which is used to separate and enrich target hybrid peptides in the supernate of the enzymatic hydrolysate. The arginine content of obtained hybrid peptide is more than 18%. The MTT method detection result show that the hybrid peptide with a high arginine content can effectively inhibit the proliferation of human cervical cancer Hela cell. The provided method reduces the product cost and the industrial production is promoted.
URL: https://worldwide.espacenet.com/publicationDetails/biblio?CC=CN&NR=107574209A&KC=A&FT=D&DB=EPODOC&locale=en_EP&date=20180112