MIPs logo MIPdatabase     MIP2018 Conference website
Custom Search
Reference type: Journal
Authors: Zhang J, Tian YL, Wang LL, Han YT
Article Title: Preparation of a novel lysozyme molecularly imprinted polymer using uniformly sized functionalized poly(glycidyl methacrylate) microspheres as the matrix and its application to lysozyme purification.
Publication date: 2014
Journal: Biomedical Chromatography
Volume: 28
Issue: (4)
Page numbers: 534-540.
DOI: 10.1002/bmc.3065

Abstract: A novel lysozyme imprinted polymer based on uniformly sized functionalized poly(glycidyl methacrylate) microspheres has been synthesized in aqueous solution using the surface imprinting technique. The microspheres were modified with hydroxyl ethyl methacrylate to allow for the introduction of polymerizable double bonds, with β-cyclodextrin and acrylamide being grafted onto the surface as functional monomers. The selective recognition properties of the resulting molecularly imprinted polymers (MIPs) were investigated by HPLC. Various factors were also investigated in terms of their influence on the retention behaviors of the imprinted polymers, including the pH and salt concentration of the mobile phase. The binding capability properties of the MIPs were evaluated, and the PGMA/EDMA-MIPs showed a high adsorption capacity for lysozyme. Furthermore, this MIP was used to separate and enrich lysozyme from egg whites. The results revealed that the lysozyme surface-modified MIP could be used to efficiently separate and purify lysozyme from egg whites. Copyright 2013 John Wiley & Sons, Ltd
Template and target information: protein, lysozyme
Author keywords: Surface imprinting technique, lysozyme, poly(glycidyl methacrylate) microsphere


  SMI logo cufflinks  Periodic table Genius shirt    Shirt featuring the template Lysozyme

Molecules Special Issue call     Sensors Special Issue call






Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:
US listings   UK listings

Searching for Molecular Imprinting books on Amazon.com:
Molecular Imprinting
Imprinted Polymers
Polymer Chemistry
Organic Chemistry
Biosensors

Find what you need at Amazon.com

Lab supplies from Amazon