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Reference type: Journal
Authors: Wang XJ, Dong SH, Bai Q
Article Title: Preparation of lysozyme molecularly imprinted polymers and purification of lysozyme from egg white.
Publication date: 2014
Journal: Biomedical Chromatography
Volume: 28
Issue: (6)
Page numbers: 907-912.
DOI: 10.1002/bmc.3207
Alternative URL: https://www.researchgate.net/publication/262573899_Preparation_of_lysozyme_molecularly_imprinted_polymers_and_purification_of_lysozyme_from_egg_white

Abstract: Molecular imprinting as a promising and facile separation technique has received much attention because of its high selectivity for target molecules. In this study, lysozyme molecularly imprinted polymers (Lys-MIPs) were successfully prepared by the entrapment method with lysozyme as the template molecule, acrylamide as the functional monomer and N,N-methylenebisacrylamide as the cross-linker. The removal of the template lysozyme from the molecularly imprinted polymers was investigated in detail by two methods. The synthesized Lys-MIPs were characterized by scanning electron microscopy and Fourier transform-infrared, and the adsorption capacity, selectivity and reproducibility of the Lys-MIPs were also evaluated. The maximum adsorption capacity reached 94.8 mg/g, which is twice that of nonmolecularly imprinted polymers, and satisfactory selectivity and reproducibility were achieved. Using the Lys-MIP column, lysozyme could be separated completely from egg white, with purity close to 100% and mass recovery of 98.2%. This illustrated that the synthesized Lys-MIPs had high specific recognition and selectivity to the template lysozyme when they were applied to a mixture of protein standards and a real sample. Copyright 2014 John Wiley & Sons, Ltd
Template and target information: protein, lysozyme, Lys
Author keywords: molecularly imprinted polymer (MIP), Protein separation, lysozyme, molecular recognition

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