Abstract: A uniformly sized molecularly imprinted polymer microspheres (MIPMs) for chloramphenicol (CAP) has been prepared by a two-step swelling and polymerization method using 2-(Diethylamino) ethyl methacrylate (DEAEM), ethylene glycol dimethacrylate (EGDMA) and polystyrene microspheres as the functional monomer, cross-linker and seed particles, respectively. The porous surface of MIPMs could be clearly observed under scanning electron microscope (SEM). Furthermore, the MIPMs had a larger pore volume and pore diameter measured using BELSORP-miniII than the non-imprinted polymer microspheres (NIPMs) did. The selectivity of MIPMs was assessed by chromatographic method, and CAP, thiamphenicol (TAP) and florfenicol (FF) could reach a stronger separation on the MIPMs with a MeCN/water/H3PO4 mixture (30:70:0.1,V/V) as the mobile phase.
Template and target information: chloramphenicol, CAP
Author keywords: chloramphenicol, Molecularly imprinted polymer microspheres, Thiamphenicol, Florfenicol