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Reference type: Journal
Authors: Lü YK, Zhang JQ, Wang XH, Li P, Liu XH
Article Title: On-line Solid-Phase Extraction Using Molecularly Imprinted Polymer Microspheres in Combination with HPLC for the Determination of Amphenicols Residues in Milk.
Publication date: 2013
Journal: Food Science
Volume: 34
Issue: (16)
Page numbers: 238-242.
DOI: 10.7506/spkx1002-6630-201316048
Alternative URL: http://www.spkx.net.cn/EN/abstract/abstract31704.shtml

Abstract: Molecularly imprinted microspheres (MIMs) were synthesized using florfenicol as template molecule, methacrylic acid as functional monomer, ethylene glycoldimethacrylate as crosslinking agent, 2,2-azobisisobutyronitrile as initiator by emulsion-suspension polymerization. An analytical method was established for the determination of amphenicols residue milk using on-line solid-phase extraction with the microspheres coupled with high performance liquid chromatography. Some extraction and detection conditions were investigated. Under the optimal conditions, the best separation and cleanup were obtained. The average recoveries for spiked milk were in the range of 80.6%-96.7% and the detection limits for thiamphenicol, florfenicol and chloramphenicol were 6.8, 10.9 μg/kg and 27.8 μg/kg, respectively.
Template and target information: florfenicol, amphenicols
Author keywords: molecularly imprinted, polymers microspheres, On-line solid-phase extraction, amphenicols, milk


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