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Reference type: Journal
Authors: Li ZW, Liu J, Huo YX, Cui Z, Liu FF
Article Title: Use of Molecular Imprinted Monolithic Column for the HPLC Determination of Melamine in Dairy Products.
Publication date: 2010
Journal: Food Science
Volume: 31
Issue: (12)
Page numbers: 203-205.
DOI: 10.7506/spkx1002-6630-201012047
Alternative URL: http://www.spkx.net.cn/EN/abstract/abstract9585.shtml

Abstract: A molecular imprinted monolithic column was prepared for providing stationary phase for the HPLC analysis of melamine in dairy products, in which the mobile phase was a mixture of 10% aqueous methanol solution and 0.1% aqueous acetic acid solution (5:95, V/V) at a flow rate of 0.5 mL/min and the detection wavelength was set at 240 nm. There was a good linear relationship between HPLC peak area and melamine concentration over the range from 1.0 to 100.0 μg/mL (r = 0.9987). Average recoveries for melamine in a blank sample spiked at 3 levels were between 87.0% and 100.5%, with a RSD of less than 5% (n = 6). In conclusion, this method is easily operated, highly selective and has no requirement of complicated sample pretreatment and is applicable for the determination of melamine residue in dairy products.
Template and target information: melamine
Author keywords: Molecular imprinting technique (MIT), high performance liquid chromatography (HPLC), Melamine, Dairy products

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