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Reference type: Journal
Authors: Zhao C, Jia GF, Lu WZ, Ni Y
Article Title: Preparation and Fluorescence Microscopic Characterization of Surface Molecularly Imprinted Polymer of Rhodamine B.
Publication date: 2014
Journal: Food Science
Volume: 35
Issue: (20)
Page numbers: 236-241.
DOI: 10.7506/spkx1002-6630-201420047
Alternative URL: http://www.spkx.net.cn/EN/abstract/abstract34772.shtml

Abstract: Poly (methacrylic acid) was grafted onto the surface of bulk SiO2 by solution polymerization to produce a molecularly imprinted polymer with a two-layer PMAA/SiO2 bulk structure (surface molecularly imprinted polymer, SMIP). The morphology of SMIP was characterized by scanning electron microscopy (SEM) and fluorescence microscopy (FM). The SEM image demonstrated that imprinted cavities were formed on the surface of SMIP. The FM image showed that rhodamine B (Rh B) could be combined and eluted quickly and effectively. SMIP had better binding property and selectivity as detected by a fluorescence spectrophotometer. The Kd of Rh B was 163.6, which was higher than that (50 and 54.5, respectively) of Rh 6G and butyl Rh B. Compared with the traditional detection method (HPLC-UV), the molecular imprinting method had better sensitivity and lower detection limit (0.1 mg/L) for trace-level determination of Rh B.
Template and target information: rhodamine B, RhB
Author keywords: surface molecular imprinting polymer (SMIP), rhodamine B (Rh B), fluorescence microscopy, silica gel, fluorescence microscope


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