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Abstract: Chemiluminescence imaging technology, which combines the advantages of high sensitivity of chemiluminescence and high resolution of the imaging assay, has become a simple, economical and speedy method for chemical and bio-analysis. However, it suffers from the disadvantage of weak selectivity of CL analysis, which has limited its usage widely. Molecularly imprinted polymer (MIP) can produce artificial binding sites in macroporous polymer particles, on surfaces or in membranes; these artificially generated recognition sites have their shapes, sizes and functionalities complementary to the analyte, and are capable of rebinding the analyte molecules in preference to other closely related structures. A precipitation polymerization method for preparing MIP microspheres with uniform shape and lowdisperse phase distribution in good yield was used. The imprinted polymer microspheres were immobilized in 96-well microtitre plates. The analyte was selectively adsorbed in the MIP microspheres. After washing, the bound fraction as a fluorophore was quantified based on bis(2,4,6-trichlorophenyl)oxalate (TCPO) reacted with hydrogen peroxide (H2O2) to emit chemiluminescence. The signal was detected and quantitated using a highly sensitive intensity charge-coupled device (ICCD) camera with Alpha Ease FC software. A new molecular imprinted polymer (MIP)-chemiluminescence (CL) imaging sensor approach towards chiral recognition of dansyl-phenylalanine (Phe) is presented. Influencing factors were investigated and optimized in detail. Control experiments using a capillary electrophoresis method showed that there was no significant difference between the proposed method and the control method. The proposed method has been also applied for the determination of transresveratrol in wine samples and dipyridamole in human urine samples. The present or similar approaches could provide useful analytical systems in many instances.
Template and target information: dansyl-phenylalanine, Phe